FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6016354
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3500.022.7567081
CGTCTGA4500.018.33017516
ACACGTC4600.018.25772313
TCGGAAG4550.018.1418613
ATCGGAA4750.018.0148662
CGGAAGA5400.015.2858164
CACGTCT5450.014.85982414
ACGTCTG5450.014.58464215
GCACACG6450.012.78849311
CACACGT7000.012.4264312
CGTTGGA14700.010.9387591
GCGTGAG10150.010.78631922
AAACGGG14950.010.5333017
ACGGGAT9550.010.5217479
AACGGGA12450.010.4801228
GAAACGG15000.010.0983446
CGTGAGC11200.09.90900623
CGGGATT9450.09.83957410
CCCAAAG48300.09.3341691
GGAAACG16300.09.2993695
Produced by FastQC (version 0.11.5)