FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12187824
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8000.019.0150031
CGTCGTA550.002046584416.4306051
CACGAGT8300.014.45548130
GTCACGA12650.012.56712629
ATCGGAA12550.011.9505422
AGTCACG13500.011.88695528
TCGGAAG13650.011.4269933
CGTTGGA28550.011.2894481
ACACGTC13300.011.16373613
CGTCTGA14100.010.84974516
CGGGATT16850.010.77007110
ACGGGAT19100.010.7577229
TCACGAG17700.010.42203430
AACGGGA24250.010.1428888
ACGTCTG15000.010.09885515
AAACGGG30200.09.8337
CACCGCG7150.09.64878130
CGGAAGA15550.09.6448934
GAAACGG31450.09.6329326
GCCACCG18850.09.54751928
Produced by FastQC (version 0.11.5)