FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5366249
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2403.6379788E-1215.9945841
TCTCGTA2850.014.99522941
ACGGGAT9500.014.2042869
CGTATGC3300.013.63177244
CGTTGGA16650.013.4262961
CGGGATT9550.013.42342210
AAACGGG17500.012.7229817
AACGGGA14200.012.6704898
ATCGGAA3601.8189894E-1212.5309382
GAAACGG17250.012.1256736
ATCTCGT3601.8189894E-1111.87111240
TCGTATG3803.6379788E-1211.83833943
GGAAACG17650.011.627655
CTCGTAT3501.364242E-1011.56774842
CCCGTAA2201.0200256E-511.2902951
TATCTCG3602.237357E-1011.24652739
ACACGTC3856.184564E-1111.0990213
CGTCTGA4253.45608E-1110.58358616
CCGGCTA4905.456968E-1210.09901638
TCGGAAG4253.583409E-1010.0823073
Produced by FastQC (version 0.11.5)