FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454300
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2801.8189894E-1214.45889644
CGTTGGA14050.014.1419871
GATCGGA2601.70985E-1013.894751
AACGGGA13350.013.3089148
AAACGGG16250.013.2866247
GAAACGG17200.012.6839926
TCTCGTA3051.70985E-1012.53688443
CCCGGAC1454.7125126E-412.4573611
ACGGGAT10750.011.9251329
CGGGATT9300.011.84976910
ACACGTC4000.011.80744913
GGAAACG17500.011.72573955
GTCACGT3753.8198777E-1111.3961729
CCCCGGT1600.001077017411.2894841
CCCGACA1600.001077017411.2894841
ATCGGAA3409.931682E-1011.2773412
ACCGTGC4405.456968E-1210.73699931
CCACGAT2759.552805E-710.6736941
AACGATC4051.5643309E-1010.55220338
CGTTGTA1500.00855894610.5368521
Produced by FastQC (version 0.11.5)