FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883407
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAG2450.020.200433
GTCACGA2900.016.28996829
TCTCGTA3050.016.22638541
CTCGTAT3250.015.92050142
GATCGGA3150.015.7481851
TACGGGC900.003761573514.99680517
TCACGAT3500.014.14013530
ACGATCA3550.013.94112232
CGTTGGA12900.013.6340011
TCGTATG3650.013.55944943
ATCTCGT3700.013.37580440
ATCGGAA4100.012.6345282
CGTATGC4000.012.37299844
AACGGGA10600.012.0964798
AAACGGG13050.012.0688677
TCGGAAG4350.011.9084053
GAAACGG13700.011.8350226
AGTCACG4200.011.78344528
ACACGTC4400.011.24760413
ACGGGAT8400.010.9798049
Produced by FastQC (version 0.11.5)