FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6660767
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT3600.026.87017439
CGTCCCG3600.026.87017435
GTCCCGA3800.025.45595736
CCCGATC4000.024.18315938
GATCGGA4250.023.8656541
CTCGTAT4200.023.56719844
TCCCGAT4350.022.75453637
TCTCGTA4700.022.01732443
ACCCGTC5300.019.10034432
TCGTATG5500.018.42459745
CCCGTCC6050.017.84803433
CACCCGT5550.017.83463731
ATCGGAA5850.017.7096042
ACACGTC6300.017.49685913
TCACCCG5800.017.45376430
ATCTCGT6500.016.61240242
TCGGAAG6450.016.0621993
CGTCTGA6700.015.78074916
CCGTCCC6600.014.99730734
ACGTCTG7450.014.49404215
Produced by FastQC (version 0.11.5)