FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7368608
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA8250.032.9934941
GATCGGA8950.031.985751
CTCGTAT9200.029.83106842
TCGTATG9800.028.69331443
ATCGGAA10050.028.6884372
TCGGAAG10100.028.3233953
ATCTCGT9900.027.94902640
CGTATGC9950.027.3564144
ACACGTC10450.027.339113
CGTCTGA11450.025.5408116
CGGAAGA12150.023.544554
ACGTCTG12100.023.42512715
CACGTCT12450.022.7665914
GCACACG13800.021.51749211
AATCTCG14050.020.01384439
CACACGT16050.018.5010212
GTATGCC15350.018.33964245
CGTTGGA21600.014.2969261
ACGGGAT13050.014.135149
AACGGGA17900.013.6984138
Produced by FastQC (version 0.11.5)