FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6810493
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT5050.032.5104334
CCGTACG5450.030.53633333
GTACGTA5850.028.06456235
TCTCGTA6350.025.85361143
CTCGTAT6500.025.2569944
ACCGTAC6600.024.87613532
CGTAATC6850.024.29550738
ATCTCGT6900.024.11874442
TCGTATG7600.022.26282345
GATCGGA7450.021.523431
TACGTAA7750.021.4744136
CACCGTA7900.020.78259331
GTCACCG8100.020.54710829
ACACGTC8650.020.0181713
ATCGGAA9000.018.8066432
ACGTAAT8850.018.80527337
CGTCTGA9500.017.99035616
TCGGAAG9750.016.8970453
AATCTCG10250.016.4555641
ACGTCTG10550.016.41300215
Produced by FastQC (version 0.11.5)