FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4584398
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC2101.8553692E-915.0002833
CGTTGGA14250.012.3592961
AACGGGA12850.011.9048898
AAACGGG15650.011.7874297
CTCGTAT2106.3272073E-611.7854242
ACCGCGC2901.3544195E-811.63675231
CCGCGCC3306.2937033E-1011.5906232
AACGGGT2158.23309E-611.5099598
TCTCGTA2351.7598377E-611.48882441
GAAACGG15600.011.2484926
ACGGGAT9500.011.1299669
GATCGGA2452.7816786E-611.0593391
GGAAACG16050.010.7929465
GTCACGA2301.7383934E-510.76001529
CGTAGAA2107.897579E-510.7521351
CGGGATT8800.010.7370810
CTCGACA1700.001839632410.58797132
ACGGGTT2402.7832642E-510.3110059
CCCGAAT4001.3114914E-910.1607681
CGATCAG2453.486408E-510.10222933
Produced by FastQC (version 0.11.5)