FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6061188
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC3550.015.20552931
CGTTGGA16100.013.9092621
CGCGCCC3151.8189894E-1213.5663133
AAACGGG17050.013.4518937
GAAACGG17100.013.1540176
CCGCGCC3950.013.09632432
AACGGGT3450.013.0352248
AACGGGA13750.012.9190928
CACCGCG4300.012.55340330
CGCCCGG5250.011.99359335
GGAAACG18400.011.5331495
CGGGATA2351.7726088E-611.48209110
CGGGAAT5100.011.46332910
GCGTGAG14300.011.32271722
ACGGGAT10950.011.2942199
ACGGGTT2851.3392673E-711.0456389
CGTGAGC15150.010.68744923
ACGCCCG3257.291055E-810.37958434
CGGGATT10100.010.2410410
GCCACCG12600.010.17422928
Produced by FastQC (version 0.11.5)