FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6149501
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA17700.014.4286621
ACGGGAT12100.014.124359
CGGGATT11800.014.102310
AACGGGA16100.012.9896458
AAACGGG18650.012.5399197
ACCGCGC3803.6379788E-1211.83550131
GAAACGG20150.011.6085976
GGAAACG20400.011.509395
CACCGCG4051.2732926E-1111.10500330
CCGCGCC4203.0377123E-1010.17289432
CGCGCCC3104.2564898E-710.15578733
CCCGTAA2901.8662286E-610.1313251
CGCAATA2109.3325845E-49.6859911
CCACGAA4152.539673E-89.2580971
GCGTGAG13850.09.25491322
CGTGAGC14400.09.21383323
CGTCAAT2000.006796659.0402581
CCGTAAA2801.4474959E-48.8691142
CAAAGCG10200.08.8629991
CCCCGAC2902.0928044E-48.57265951
Produced by FastQC (version 0.11.5)