FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7998120
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT18000.012.9951979
AACGGGA21900.012.7350218
CGTTGGA26450.012.2850911
CGGGATT18250.012.07772710
AAACGGG27000.011.745667
GAAACGG28150.011.106026
CGTGAGC22200.010.94210823
ACCGCGC6200.010.88382331
GGAAACG28950.010.8280915
CGTATGC4405.456968E-1210.73473644
GCGTGAG21500.010.67067522
CGCGCCT2453.501252E-510.09856133
CACCGCG6850.09.85105130
CCGCGCC7100.09.82043732
CTCGTAT4601.5279511E-109.77905642
CCGGATC1900.00453319039.4963652
CGCGCCC5252.0008883E-119.42532433
ACAGGCG29050.09.29100318
TCTCGTA4702.3283064E-99.09244241
AGGCGTG27100.08.96358920