FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2706002
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG99410.3673685385302745TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12450.041.977645
CTCGTAT13400.040.11746241
TCTCGTA13500.039.9869140
TCGTATG13650.039.05314642
ATCTCGT13700.038.91061839
CGTATGC13700.038.7478743
ATCGGAA14250.037.8461841
TCGGAAG14350.037.5824432
CATCTCG14150.037.352538
ACACGTC14700.036.7192812
ACGTCTG14850.036.04547514
CGTCTGA14900.035.92451515
CACGTCT15200.035.3634413
GCACACG15600.035.03336710
CGGAAGA15550.034.682193
GTATGCC15400.034.6165744
CACACGT16300.032.97695511
CTTAGGC17300.030.6807632
ACTTAGG18450.028.6465131
GCATCTC18850.028.39656637
Produced by FastQC (version 0.11.5)