FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7970797
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24300.014.7701251
ACGGGAT15000.014.0984959
AACGGGA20350.013.9297178
AAACGGG24300.013.7948227
CGGGATT15000.012.44867110
GAAACGG26350.012.3800826
GGAAACG26600.012.0099965
AACGGGT5350.010.5128968
CGGGAAT8750.09.77038510
GCGTGAG19850.09.52039422
CGTTGAA4304.598405E-99.4492521
ACAGGCG28150.09.27105918
CACCGCG7300.09.24564630
ATACCCG1950.0057273199.23059545
ACCGCGC7100.09.18927531
CGCCCGG8650.09.10324635
CCCCGTT3806.7063775E-78.9104791
ACCACGC15200.08.7327331
CGTGAGC21750.08.68894523
CCCAAAG91000.08.6820051
Produced by FastQC (version 0.11.5)