FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7327167
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23400.015.0460791
ACGGGAT14350.014.5804659
AACGGGA20250.014.2208398
CCCGATA1150.001175325113.7377241
AAACGGG24300.013.6097887
CGGGATT13450.012.71251810
GAAACGG26350.012.55096156
GGAAACG27100.012.1205935
CGGGAAT8600.011.510509510
CCCGTAA3002.5818917E-710.5322561
ACCGCGC5007.2759576E-129.89910531
CGGGATA4253.981768E-99.52848510
CCGCGCC5201.6370905E-119.51843632
CGAAGAT6450.09.4475371
ACGCCCG3401.4933266E-69.26393234
ACGGGAA9250.09.2423459
CGTTAGG2200.00144628149.2328211
GATTTCG9350.09.1434965
CGCCCGG6900.09.12960935
GTTGGAA44400.08.8711962
Produced by FastQC (version 0.11.5)