FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006072262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10749907
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA27250.012.80112651
CGGGATT17400.011.120831510
AAACGGG29700.010.5301447
AACGGGA24450.010.2155198
GAAACGG31100.010.0561656
GGAAACG30900.09.9762735
ACGGGAT19350.09.8838939
TATGCCG3755.662696E-89.59954245
AACGGGT6150.09.5129368
TCTCGTA3858.488678E-89.35068140
ACCGCGC7850.08.59943631
CCCGGTT3451.7868362E-58.4802231
ACCACGC20350.08.18249331
CGCCCGG10900.08.05063535
ACGCCCG6257.1304385E-107.92017434
CCCTAAA31750.07.86796241
CTCGTAT4601.2787568E-67.826368341
GTACGAT2900.00206280177.75900826
CCCGTAT3501.9431773E-47.71607071
CACCGCG9350.07.701385530
Produced by FastQC (version 0.11.5)