FastQCFastQC Report
Thu 12 May 2022
EGAF00006072336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70292500
Sequences flagged as poor quality0
Sequence length8-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG2129630.3029668883593556No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1153210.1640587544901661No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGACAATCTCGTA1001930.142537255041434TruSeq Adapter, Index 4 (97% over 39bp)
GGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGG912940.12987729843155388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)