FastQCFastQC Report
Sun 15 May 2022
EGAF00006072338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81919796
Sequences flagged as poor quality0
Sequence length8-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTGA4004797348.886807530624225No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG3830870.46763666257176717No Hit
CGCGCGCCCTCCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGA3531240.4310606437545328No Hit
NCTCCTGA2778410.3391622215465478No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCCTGAATCTCGTA1800860.21983209040217824TruSeq Adapter, Index 27 (97% over 39bp)
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1738000.212158731449966No Hit
GAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGACCGGGGTC1500200.1831303388499649No Hit
GGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGG1393960.17016155655465742No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA1301940.15892861842575878No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC1293550.15790444595345426No Hit
ACTCCTGG1155240.14102085898749064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)