FastQCFastQC Report
Thu 12 May 2022
EGAF00006072339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006072339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81919796
Sequences flagged as poor quality0
Sequence length8-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGACA4017192649.03811772187518No Hit
TTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG3523860.4301597626048776No Hit
TCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGT2790990.34069786990192213No Hit
NTAGGACA2428360.2964314022461677No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTAGGACAGTGTAGATC1822150.22243097382713212Illumina Single End PCR Primer 1 (96% over 33bp)
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG1788960.21837945006601334No Hit
TCCGTCCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGT1376390.1680167758230257No Hit
CCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGG1296440.15825723003509434No Hit
TTCCGCTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG1117670.13643466592617004No Hit
CGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGG1058710.12923738237824714No Hit
GTCGGACA927760.11325223514960903No Hit
CTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGA838950.10241114369962541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)