FastQCFastQC Report
Thu 12 May 2022
EGAF00006075695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006075695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47416287
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT2141500.4516380626766495No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1635870.3450017079574366No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG1586520.3345938917570665No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1551000.3271027948687758No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1431650.3019321188097246No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC1407640.296868457878197No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1377000.2904065432200543No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT1066880.22500285608613768No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT978110.2062814408053503No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT903800.19060961057537043No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG855130.1803452050136275No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA846870.1786031875503031No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA837130.17654904104996666No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCA823980.17377573237651442No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT796130.16790222313273917No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG790310.16667479678448882No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG788750.16634579590763823No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA770710.16254119602405814No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT753920.15900021863795452No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA749100.15798369028768533No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA729650.15388172422695182No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC699970.14762227164687106No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG684310.14431960899848612No Hit
ATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGG679500.14330518962819674No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA672090.14174243546315637No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAA671760.14167283912382259No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC658620.13890163943035017No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA652630.13763836042244304No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT634310.13377470909942824No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC627740.132389109252692No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG626930.13221828187432727No Hit
CTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAG612980.12927625480249014No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG610170.12868363142816305No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC606040.1278126226965009No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT589910.12441083798906481No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC581640.12266671154576064No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG580180.12235880046870815No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT553200.11666877248317652No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA539540.1137879058307539No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG535370.11290846117917247No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT531120.11201214468775254No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG524080.11052742278196519No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG520350.10974077324949548No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC519360.10953198423149413No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA509840.1075242352907135No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT501470.1057590190476112No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA499460.10533511407166908No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG496980.10481208703667581No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC492360.10383773828600287No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC491390.10363316722796115No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)