FastQCFastQC Report
Thu 12 May 2022
EGAF00006075719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006075719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80389043
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG9876191.2285492688350574TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT3026740.3765115104057154TruSeq Adapter, Index 6 (97% over 36bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG2407940.2995358459485579No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA1219400.151687338783222No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACA1044750.12996174117908083No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1033830.12860334710042512No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG1023450.1273121263553293No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG832870.10360491541117114No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGG806390.10031093416549318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)