FastQCFastQC Report
Thu 12 May 2022
EGAF00006075757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006075757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36368578
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACT747240.20546307859493435No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG642180.17657550427184698No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA617180.16970143842302549No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT530230.14579343740082443No Hit
CTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGG510040.1402419418213162No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA466850.12836630566089222No Hit
CTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTT454150.12487428020969091No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA449750.12366444462029832No Hit
CACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGAC408350.11228099157464996No Hit
CAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGG403290.1108896806468485No Hit
CTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGG397580.10931964400697766No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA395130.10864598555379316No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC385810.10608333380535252No Hit
CCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCT371950.10227235169876589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)