FastQCFastQC Report
Mon 3 Apr 2023
EGAF00006127029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80481094
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1132530.14072000562020193Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC956690.11887139605731502No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG898860.11168585755059442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)