FastQCFastQC Report
Sat 1 Apr 2023
EGAF00006127101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73823105
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGG7832081.0609253024510414No Hit
TAGCTCGCTCCTGAGC2521450.3415529596052618No Hit
GCGCAGTTGACGCGAA2277080.30845085695054414No Hit
GGTATACTGCGAGTGA2132840.2889122585672873No Hit
CCTACGGGAAGACAAT2111650.28604188349975257No Hit
CGCCCTTCTTATGACC1963300.2659465488480876No Hit
CTCCAACTGAAATCGA1808020.2449124837000557No Hit
CACATTACTGAGCCTC1683170.22800043428138117No Hit
TTCCCTGGATGATGAA1628090.22053935553103596No Hit
GCCATTCACTAACGGG1458740.19759938301159236No Hit
CCCACAGGATAGCGAG1418840.19219457106281293No Hit
GGGTGAAACACCAAGG1403150.190069220198744No Hit
GAGGTGCCTCACAGTT1392990.1886929573065235No Hit
CAAGGGAACATGTCCA1363070.1846400256396693No Hit
CAACACCCTGGAATGC1328780.179995138378425No Hit
GTCCTGACTCTGGCAC1318530.17860668418105688No Hit
TATTGGTCTATGTCAC1317090.1784116232986949No Hit
CTCAAACGAGCGAGTA1285120.1740810007923671No Hit
GGGATCAGAGCGTATT1275210.17273860263666777No Hit
GTAAGAAACCGGCACT1256420.17019332903973627No Hit
GGAGTTACTGGACTGA1242180.16826439364749016No Hit
ACAGTCATGATCCTCC1231110.16676486311433256No Hit
TCAGTCCTGCGACAAG1227600.16628940221357527No Hit
TAACGTTCTCTGTACA1220500.16532764369637393No Hit
CCTATCCGACGATTGT1207130.1635165575872215No Hit
GTGAGATCTTCGCTAA1177680.15952729162502713No Hit
TCGACTCTGTCAGGTT1151740.15601348656359007No Hit
CGGTTGGACCGAAAGC1127470.15272589794211444No Hit
ACAATGCACAAGCTAA1124730.15235474042984237No Hit
TCTATGGGAAGACAAT1115330.15108142633664623No Hit
ACCTGCTACTTAGCTG1103780.14951687550936796No Hit
GAACTAGCTCATCCTC1098250.1487677875375196No Hit
ACACATCGATTCCATT1080030.1462997255398564No Hit
CCTGCTATGTGTGCTA1066480.14446425682040875No Hit
TGCCTACTGGGGATTG1047020.14182822572960052No Hit
GTCGCATCTGGATGGT1044120.14143539478595488No Hit
CCGACTACTTTCTGGA1041740.1411130024942733No Hit
GTACCCTTGGGATGGT1038450.14066734256165467No Hit
GTGAAGCGAGTCTTGA1036780.14044112612169318No Hit
ATGGGACGAGAAACAT1036710.1404316439954673No Hit
CTTTCTGCTCGGGTAA1020770.1382724283948772No Hit
GCCATAATGTGGTGAG1006980.13640444952836922No Hit
CGTTACCTGGACAAAT997680.1351446813297815No Hit
TTTCGCTCTATCCTCC977610.13242602028186162No Hit
GGTCTAAACATCGGTT976710.13230410723038538No Hit
GTCGAATACCCTATCC974530.13200880672792076No Hit
GTCCGAGTGTGGTGAG966660.13094274482223417No Hit
ACGTCCACTCCGATTA965210.13074632935041136No Hit
TGCGCAGACCAACGCC961360.13022481240798528No Hit
CTGTAACGAAGCAGGT936710.12688574938699745No Hit
TCCATTCCTCCGATTA936610.126872203492389No Hit
CTAACAGTGATCACAC928240.12573841211366008No Hit
GTTGCTAGACAAGTTC920140.12464119465037402No Hit
CTGCCATTGGAAGGTC919840.1246005569665486No Hit
GAAGTATGACATCTTT901920.12217313265271083No Hit
CGCAATCCTGGTTTGT898680.1217342456673964No Hit
TGGTCAAACTGAGTAA898210.1216705799627366No Hit
CAGTATTCTATTGCCA897850.12162181474214612No Hit
GGTAGTTTGTAAAGCA893210.1209932852323131No Hit
GCAACTTGACCCGCAA887360.12020085039771762No Hit
TTTAGGGACCCGGACT871270.11802131595521483No Hit
GATACGGGAAACGTTC868960.11770840578975918No Hit
ACACACTGATCCCGTT868920.11770298743191579No Hit
ATTTCTGACAGCGCTC868150.11759868404343056No Hit
GTTGACTGAATTGCCA866330.11735214876155643No Hit
CTAACAGTGGCTGAGA864820.11714760575296854No Hit
AAACCCTGACAAGTAA859220.11638903565489422No Hit
CGATCGGTGTTGCGTA855850.11593253900658879No Hit
TTCGATGGAAACGTAA855850.11593253900658879No Hit
TAGGACTTGGCCCTAG854850.1157970800605041No Hit
ACCCTTTCTGAGCCTC837790.11348615044029914No Hit
CTGCCTTGAATAACCC837160.11340081130426578No Hit
CACCGTTACACGCCAC830590.11251084602848932No Hit
TGGATAGCTCGTGCAC829230.11232662186181414No Hit
GTGTACTGAAAGTCTC827260.11205976773802728No Hit
GCTAGATACCAATAGA825240.11178614066693618No Hit
TCTGAACGAGTTAGTC820900.11119824884092859No Hit
GCTAAGACTGATAGCG820460.11113864690465132No Hit
CTCTTTCTGCTCATCC819560.1110167338531751No Hit
TAATTGCCTTACGTTG813460.11019043428205844No Hit
AGCGCCTGATTTCTGT812580.11007123040950391No Hit
CATAGCATGCTGCGGT810200.10974883811782232No Hit
GGTGAGATGTCGTACA806620.1092638950908391No Hit
TAGGACTTGGCACTTA802490.1087044496435093No Hit
CGATGATTGGCGGTTC788070.10675113164096796No Hit
CAATGTGACCAAGTAA788050.10674842246204627No Hit
TTAGCGATGTACGATC787950.1067348765674378No Hit
CTAATCACTCAGGGTA785390.10638810166546098No Hit
CAAGGGTGACGATCGC782140.1059478600906857No Hit
TTGCGATGAGGTATGG781290.1058327199865137No Hit
GAAGTTCTGGAAGGCT777260.10528682043379238No Hit
CTGTCGTTGTGCTCAA770300.10434402616904287No Hit
GGCTTCTACATTGGTC768690.10412593726584651No Hit
CATCAGTGAGGCAGTA753190.1020263236015337No Hit
GTCTACCACAAATCGA746790.10115938634659162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)