FastQCFastQC Report
Sat 1 Apr 2023
EGAF00006127119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78409270
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTGGTCGC2062650.2630620078467763Illumina Single End PCR Primer 1 (100% over 16bp)
TTGACGTGATTGTTTC1710830.21819231323031066No Hit
CGAATTTCTTTACGAC1424030.18161500547065418No Hit
CCGTACGGAACCTAGT1391240.17743310197888593No Hit
GTACATCGACGAATCT1167000.14883444266220053No Hit
ATAGGAAACAGTCTGT1145230.1460579852356743No Hit
GGACACAGAGATCCAT1142120.14566134846045628No Hit
AAGACCTACGTCCCGA1118590.1426604277785012No Hit
GTGTACTGAACCGTGC1109230.14146669137462956No Hit
TCGGGTGACAACGAAT1057530.13487308324640698No Hit
GTAGGCGCTGCGCACT1053640.13437696843753294No Hit
TGAGCCCGAATCCTCC1035080.13200990138028323No Hit
ACAGCCGACCGTTCTC1011280.1289745459943703No Hit
TCAATCGGAGCTTCGG977950.12472377309468638No Hit
CCCATTTCTAATACAG969950.12370348557000978No Hit
CCTACTGTGAGTCGGA943730.12035949320788218No Hit
TGTCAATCTTATTGCG928350.11839799044169139No Hit
GATTCTGGAATCGGTT921260.11749376062294675No Hit
GGTTCAATGTCCTACC917210.11697724006357922No Hit
CTATGTTACTATGACC875130.11161052768378026No Hit
GTTGATTCTGAATTGA842210.10741204451973599No Hit
AATGATGGACACTCAA828390.10564949782085716No Hit
ACTCGCTACGAATTGA826670.10543013600305168No Hit
TACCCGGGACGATTCA819820.10455651481004734No Hit
TATCACTACGGTTTGT790920.10087072612715309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)