FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93626284
Sequences flagged as poor quality0
Sequence length31
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2650930.2831395081321395No Hit
CCTCTGCAAAAAAAAAAAAAAAAAAAAAAAA2269830.24243512644376658No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2262370.24163834164346415No Hit
CATCATCTTACAAAAAAAAAAAAAAAAAAAA1723170.18404767618460646No Hit
TCCGAATTCAAAAAAAAAAAAAAAAAAAAAA1520690.16242127050561997No Hit
GTAGTCACAGCGAAAAAAAAAAAAAAAAAAA1376050.146972617219327No Hit
GTGCCACTCAAAAAAAAAAAAAAAAAAAAAA1276760.1363676892270978No Hit
CCTCTGCAATAAAAAAAAAAAAAAAAAAAAA1251150.13363234623303002No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1226350.1309835174062873No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1185440.12661401791830165No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1131680.12087204059065294No Hit
TTGCTCGAAAAAAAAAAAAAAAAAAAAAAAA1111320.11869743756998836No Hit
ATATGGGTGTAAAAAAAAAAAAAAAAAAAAA1108850.1184336227634539No Hit
CATTTACGAGACCCGAAAAAAAAAAAAAAAA1058520.11305799555176192No Hit
CATCATCTTAAAAAAAAAAAAAAAAAAAAAA1037280.11078940183079358No Hit
CTGAGGCCCAAAAAAAAAAAAAAAAAAAAAA1018880.10882414173353286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGCG269750.024.8813951
AGGGCGC271150.024.8558252
GTGCGCG1303550.024.8155653
GCGCAAC347450.024.7906281
AGGCTTC1343750.024.7839723
CGCGGCG259150.024.7575974
CGGCTTC401500.024.7387261
AGCGCCA338550.024.7332481
CCGCGGC260200.024.7296713
AAGCGCG230650.024.6998332
CGGCATG203350.024.695972
CAGCACC974450.024.6690851
GGGCGCG273300.024.660463
CGTGCGC1407100.024.6488632
TCCGCGG260800.024.6342582
AGCGCGC240850.024.6246173
AAGGCCG205350.024.6015572
GGCTTCA1343600.024.5960854
CCCGTTG150650.024.5915661
GCGCCAT534650.024.5871492
Produced by FastQC (version 0.11.5)