FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37581667
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC532050.024.9751011
CCCGGCG538600.024.9215742
TCCCGCA330600.024.843764
CCCGCAT330000.024.8396825
GCAATAG185950.024.7928241
GCGCCTG155000.024.7687823
CTCCGTA289100.024.7563292
GGCGCCT134250.024.7513892
GAGGCGA292600.024.7505025
GTATACT406650.024.7435651
AGTTCCC333650.024.7269291
GCCGATG156200.024.7065533
CCGGCAA206400.024.6877233
GGGCCCG333100.024.6873916
GGCCCGA333700.024.6350297
GGGCGAA403000.024.620138
GGCAATC206450.024.5908155
GGGCTGA396800.024.5899563
GGGTAAG176700.024.5851634
GTCGGCC49450.024.5730382
Produced by FastQC (version 0.11.5)