FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52948067
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCT199500.024.7644272
GAGGCGA298600.024.742935
GCTTACA441900.024.6772162
GTCGCTA166350.024.6493153
TGGGCCG114600.024.6260011
GCCGATG160700.024.613563
ATGGCCC216350.024.586493
GTGCCCG263250.024.585151
CGCGGCA23500.024.5769255
GACGCCA298250.024.5715310
GCGCCTG221550.024.5680393
GTATACT302150.024.5402551
TATAGCG474650.024.5389773
GGCCGTA101750.024.523363
CCCGGCG59800.024.5217292
GCGTATG135600.024.5139053
GGGCGAA300250.024.4865368
GGGCCGT102250.024.476822
GGCCCGA136900.024.4688197
TGCCCGC264700.024.4641462
Produced by FastQC (version 0.11.5)