FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53941734
Sequences flagged as poor quality0
Sequence length31
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCA401650.024.8313064
CGCGCAT361400.024.8260775
AGCGCGC402750.024.8193593
AGCATAA250750.024.7041231
TGCGTAA221300.024.6183623
AAACCCC835750.024.56170725
AAGCCTA279100.024.554585
GTCCGAA152000.024.5501464
GGCCATA167600.024.5382881
GGTAAAT396200.024.509661
GGCGCTA45800.024.5072698
GCGCATC62100.024.4992223
GTCGCTA74400.024.4984113
GGGCGCG37150.024.4977323
AAGCGCG368100.024.496512
GCCGATG143300.024.4877873
TCGCCCA129200.024.4663371
CCCGCAT36950.024.4611665
AGCGCCA98500.024.424361
GCGCCAT99600.024.412622
Produced by FastQC (version 0.11.5)