FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48871222
Sequences flagged as poor quality0
Sequence length46
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4313650.8826564639615518No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3250330.6650805662277076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080190.6302666219395946No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT708400.14495238117843667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT29300.013.5827671
TCTTCCG210350.011.0068272
ACGCTCT68100.010.7775921
GACGCTC47200.09.403882
CTTCCGA251900.09.3338743
GGTCCGA21000.08.951821
TTCCGAT269050.08.66484454
CCTTCCG46950.08.5617641
GCTTCCG45850.08.4182291
CGCTCTT98700.08.2851862
TCCGATC278500.08.2132695
CTCTTCC280350.08.0323121
CGTAATC12050.07.9663061
TATACGG29550.07.91609763
GTATACG32000.07.8725732
GGATACG13100.07.32778551
CCGATCT312950.07.27130376
TCGACGA11300.07.2561821
CGGGAAT42600.07.2295941
CGCGAAC7204.9112714E-117.221771
Produced by FastQC (version 0.11.5)