FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40757960
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGCC277850.024.876342
GCGCGCA113850.024.8155924
TGCGCCA76200.024.7612761
CAGCTAA511550.024.7214321
CGCGCAT100950.024.7174385
AGCGCGC115050.024.6979833
GGCGCCT102400.024.6971132
ATGCGGC113000.024.6887997
TGGGCCG72100.024.677911
GTCGCTA51250.024.6607323
GGGCGCG29250.024.6603153
GCTAAGC511550.024.623443
GAGCGAA101550.024.5590865
TCGCGCG84600.024.5405047
CGGCATG50650.024.5333712
GTGCCCA321500.024.52100610
AGACCAG524700.024.5141261
TGCGGCT113950.024.4940268
GGGCCGT63450.024.4900532
ATTAAAC239100.024.4794145
Produced by FastQC (version 0.11.5)