FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40757960
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2825950.6933492255255169No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2594980.6366805404392173No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1576730.3868520406811332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT53500.011.2511281
CGACGCT22600.010.7953011
TCTTCCG162550.010.467292
TCGACGA8850.010.3943641
GGTCCGA17400.010.1138561
GGTATTC40550.09.52025120
CTTCCGA187000.09.0984723
CGCTCTT78600.08.9793282
GCGACCG12400.08.8700151
TTCCGAT203750.08.301624
TATTCCG13100.08.2433891
CGCGAAC6151.0913936E-118.1292091
TCCGATC210050.07.8815975
GACGCTC33500.07.69903952
GCTTCCG39150.07.6620121
CCCGATC30100.07.64037561
CGCGATC11100.07.5667551
GGTACGG9150.07.4308961
CGTAATC10050.07.163411
TCGATCT38500.07.06417661
Produced by FastQC (version 0.11.5)