FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59661404
Sequences flagged as poor quality0
Sequence length46
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21378633.583326667940969No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5813160.9743585652124446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4096250.6865829037479574No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1574140.2638456178470088No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1396570.23408265752512292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT30900.015.0711861
ACGCTCT43850.012.7625781
TCTTCCG144450.011.373852
TCGACGG5950.011.0852881
GACGCTC40600.09.9444012
CGCTCTT69900.09.6647251
CTTCCGA167550.09.102953
TTCCGAT166150.09.0842974
CGCGAAC6300.08.883161
TCCGATC159850.08.8803145
TTCGCGG132800.08.66072514
CTTCGCG135750.08.56081913
TCGCGGG138100.08.48776115
CGACGTA21850.08.23841940
TGTCGCG9500.07.99484351
CCGATCT171450.07.9885336
CGGGAAT41550.07.98522231
CGTTACG9600.07.9115641
CTCTTCC229300.07.87978461
GGATGCG144400.07.785211624
Produced by FastQC (version 0.11.5)