FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73684598
Sequences flagged as poor quality0
Sequence length31
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCAAAAAAAAAAAAAAAAAAAAAAAA1384780.1879334403100089No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1292890.17546272017389578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1163640.15792174098581632No Hit
CCTCTGCAATAAAAAAAAAAAAAAAAAAAAA838920.11385282986819037No Hit
CATCATCTTACAAAAAAAAAAAAAAAAAAAA799110.10845007256468985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCCG123050.024.758621
TGGCGCG98500.024.748442
GTCGCTA147950.024.7063163
GAGCCGG128450.024.6906193
GGGCCGT100200.024.6155072
GCGCATC106000.024.589283
TGCGGGC99150.024.5608986
GTGCGCA161800.024.553933
TCGCCCA299350.024.5406151
TACGCGC71150.024.4926556
CCCGGCG108550.024.472512
AAGGCCG116500.024.4657422
TGCGCAA162850.024.4494154
GGCCGTA100800.024.4315663
GGCGCCT142650.024.4238782
AACGAGA129100.024.411445
AACGACC86800.024.40775312
GAGGCGA29400.024.4068285
GAGGGCG30650.024.4006521
CTGGCGA98400.024.3883699
Produced by FastQC (version 0.11.5)