FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28796199
Sequences flagged as poor quality0
Sequence length31
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA853380.2963516122388236No Hit
GTAGTCACAGCGAAAAAAAAAAAAAAAAAAA628980.21842466083805018No Hit
TCCGAATTCAAAAAAAAAAAAAAAAAAAAAA618850.2149068354472755No Hit
CATCATCTTACAAAAAAAAAAAAAAAAAAAA573830.19927282763950896No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA568960.1975816322147239No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA495420.17204353949630644No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAA476730.16555309956011904No Hit
CCTCTGCAAAAAAAAAAAAAAAAAAAAAAAA473590.1644626778693952No Hit
CTGAGGCCCAAAAAAAAAAAAAAAAAAAAAA456700.15859732043107494No Hit
ATATGGGTGTAAAAAAAAAAAAAAAAAAAAA444500.15436065016775302No Hit
GGCTCAAAAAAAAAAAAAAAAAAAAAAAAAA404160.1403518568544411No Hit
TACGATGGGACAAAAAAAAAAAAAAAAAAAA374890.13018732090301222No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA367660.12767657286991246No Hit
CATTTACGAGACCCGAAAAAAAAAAAAAAAA343210.11918586894055011No Hit
CATCATCTTAAAAAAAAAAAAAAAAAAAAAA319720.1110285423433836No Hit
TACGATGGGACAATCAAAAAAAAAAAAAAAA317590.1102888613875741No Hit
CCTCTGCAATAAAAAAAAAAAAAAAAAAAAA316900.11004924643005837No Hit
GTGCCACTCAAAAAAAAAAAAAAAAAAAAAA312300.10845181337995338No Hit
GGACTGACAAAAAAAAAAAAAAAAAAAAAAA292760.10166619559755091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCCG146350.024.8185881
GAGGCGA166950.024.806745
TGGCGCG56950.024.6948012
GGGCCGT129750.024.6552622
GGCCGTA129350.024.6536773
TCCCGCA117550.024.5761724
ATCGCGA28250.024.5564849
GTATACT202000.024.5546911
GACGCCA167500.024.52143910
GTGCGCA42400.024.5003813
GGGTAAG37400.024.4667574
GGGCGAA192100.024.4588838
CGCCAAT170800.024.45748312
TTCGGCA27250.024.455941
CCCGCAT117700.024.4491355
GCTTACA183850.024.4445482
GTCGCTA82400.024.440273
ACGCCAA168350.024.38277811
GGGAATC112650.024.3775758
GCGCCTG106450.024.3674533
Produced by FastQC (version 0.11.5)