FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81214874
Sequences flagged as poor quality0
Sequence length46
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49698456.119377837118851No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4840150.5959684182973675No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2921210.35968903922697704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2598520.3199561696050898No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2528700.31135922220355844No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870280.10715771103702015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT34550.020.539061
GACGCTC45950.014.9628342
ACGCTCT57750.011.2836843
TCTTCCG143850.010.3372252
CGTAATC36200.09.2216411
CACGACG15400.09.2146442
CTTCCGA168150.08.879923
CGTTCGA13150.08.5126071
TTCCGAT170900.08.445354
TACGTCG5006.726623E-98.0012821
TCCGATC182250.07.99701645
ACGACGC20300.07.77886773
CGTCCAA19550.07.7708251
CCTTCCG35850.07.75043251
CCCGATC19600.07.6490151
CGCGAGA13500.07.4034911
GCTTCCG30700.07.22744661
ACACGAC22200.07.20339631
CGTAGCG10100.07.129879529
CGGAGAA52250.07.11585241
Produced by FastQC (version 0.11.5)