FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24271971
Sequences flagged as poor quality0
Sequence length46
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20765588.55537442756503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2998961.2355650886366005No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1727170.7115903360299829No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1579350.6506888130345904No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1336720.5507257733622045No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455360.1876073434662558No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433830.17873702963801333No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294640.12139104813531626No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG258930.10667860471652672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT15250.015.4724881
GACGCTC19300.012.2246642
ACGCTCT26000.011.4593751
TCGACGG2101.8801402E-611.42641451
TCTTCCG79100.011.0463082
CGCGAAC2652.4780275E-710.5640431
TTCGCGG35950.09.62514114
CGCTCTT37400.09.6238521
CTTCCGA83650.09.4659413
GATCGGA86950.09.38347440
CTTCGCG36800.09.239861513
CGTAATC10550.09.0978091
GATGCGT36550.09.08345425
TCGCGGG39950.09.06202615
CCCTTCG39350.09.0478611
GGATGCG38000.08.84231324
TTCCGAT89000.08.7177254
CTCTTCC99800.08.4753541
TCCGATC91150.08.2714555
GGTCGGA8500.08.233741
Produced by FastQC (version 0.11.5)