FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82944706
Sequences flagged as poor quality0
Sequence length31
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCAAAAAAAAAAAAAAAAAAAAAAAA1260010.15190963483552525No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA886250.10684828999213043No Hit
CCTCTGCAATAAAAAAAAAAAAAAAAAAAAA874890.10547870288430464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA633350.024.901582
GCAATAG633450.024.8946511
GGCGCCT992650.024.8045222
TTATCCG1359900.024.7689041
GCGCCTG1158350.024.712333
CCCGGCG364500.024.7004322
AACGCTA578450.024.6426775
CGGCAAT239600.024.6258874
TAGCATA630550.024.5969815
ACGCTAC578350.024.5433226
CAATAGC645000.024.5138262
GTCGCTA187050.024.5136833
GGCAATC240250.024.4862965
TATCCGC1453800.024.4768492
AACCGGA421350.024.4525535
ACCGGAA417450.024.4505526
AACGTCA214200.024.423655
AATAGCA642700.024.3577423
GCCGTGC590300.024.350721
GGCCGTA187750.024.335733
Produced by FastQC (version 0.11.5)