FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52165146
Sequences flagged as poor quality0
Sequence length31
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA942700.1807145330332249No Hit
CCTCTGCAAAAAAAAAAAAAAAAAAAAAAAA798430.15305813579051422No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA715300.13712220799688743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATAG394450.024.8790441
CTCCGTA353400.024.7294042
CCCGGCG167150.024.7101022
AACGTCA200450.024.6273335
CGGCAAT179550.024.6185654
CCGGCAA183950.024.5869263
GGCTATC130250.024.5801331
GAGGCGA56950.024.5625045
CGGCATG86200.024.5377062
GCCGACC258000.024.537171
TGGCGCG48050.024.5334822
GGCAATC179600.024.5142885
TAGCATA394900.024.5013565
GGCGGGA25500.024.4727291
GGGCGCG46650.024.4655273
ACCCGGC89050.024.4644951
GTCGCTA58150.024.4425333
ATCGCGA59650.024.4327039
ACGCTAC156400.024.402176
CAATAGC403200.024.3971982
Produced by FastQC (version 0.11.5)