FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52165146
Sequences flagged as poor quality0
Sequence length46
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20826013.9923227666227556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5342961.0242394414078704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4088880.7838337114977115No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1651370.3165657774637494No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1486770.2850121420152835No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT593910.11385188110084078No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524490.10054414493539422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT33300.016.8652671
GACGCTC41250.011.4833452
TCTTCCG158850.011.0471782
ACGCTCT47050.010.3648091
TCGACGG6000.09.9931211
CGCTCTT71500.09.3921371
CTTCCGA180500.09.0033143
TTCCGAT176100.08.8432544
TCCGATC175350.08.6993785
TTCGCGG111900.08.59816514
CTCTTCC215550.08.5118861
CTTCGCG117350.08.36910813
CGCGAAC6301.8189894E-128.248291
TCGCGGG117350.08.21596215
GGATGCG117750.07.98452324
CCGATCT188700.07.7561196
CGTGCAT124900.07.57511229
CCCTTCG137500.07.56417211
GATGCGT122950.07.50040725
GGATCGC24350.07.30503231
Produced by FastQC (version 0.11.5)