FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87717321
Sequences flagged as poor quality0
Sequence length31
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1049530.11964911696288581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC330900.024.8647371
GTCGCTA696750.024.8095363
CAGAATA424250.024.6967181
TGCGGAA368400.024.6622544
AACGAGA374300.024.6442575
GCCGACA658150.024.6407972
GAGGCCG343950.024.6017193
CTCCGTT720700.024.5921971
AGAATAT425300.024.5637262
ATTAACC768600.024.5623743
AAGCCCG455750.024.529835
TCGCTAT665150.024.5166974
AGCCCGG454900.024.5070616
CCGGGAT536350.024.5029346
CCCGGCC452500.024.487578
CTCGTTA330900.024.4830171
GCGCGCA126200.024.4666824
AAGGCCG179100.024.4642372
TGCGCCA258500.024.455771
CGGCCTA449450.024.45344210
Produced by FastQC (version 0.11.5)