FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47793961
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCT203100.024.7684422
GCGCCTG238000.024.7291283
GAGGCGA66000.024.7181195
GTGCGCA121750.024.7044933
TCGCCCA165450.024.6606541
TGGCGCA38900.024.6487315
TGCGCCA90600.024.6218621
ATGCGGC242350.024.606557
GCGCATC59250.024.6013813
GCGTATG160800.024.5824453
CCGAAAC253250.024.5778686
CCGCGGA53050.024.5310422
GGCTATC88150.024.5260141
TGCCGAA255700.024.5182274
ACCCGGC52500.024.508141
GCGGCTC243100.024.5050819
GCGCGCA83550.024.4935134
CGCAATG50100.024.484181
AGCTTAC191450.024.4733543
CGCGGCA32800.024.4686495
Produced by FastQC (version 0.11.5)