FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135540254
Sequences flagged as poor quality0
Sequence length31
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6325370.4666783345411172No Hit
CCTCTGCAAAAAAAAAAAAAAAAAAAAAAAA5796810.42768180145213536No Hit
CCTCTGCAATAAAAAAAAAAAAAAAAAAAAA3984600.29397908609496926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3975380.2932988453747475No Hit
GGCTCAAAAAAAAAAAAAAAAAAAAAAAAAA3354530.24749326498974983No Hit
TCCGAATTCAAAAAAAAAAAAAAAAAAAAAA3345200.24680490859933019No Hit
ATATGGGTGTAAAAAAAAAAAAAAAAAAAAA3323420.24519800590015128No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260070.24052411765437595No Hit
CATCATCTTACAAAAAAAAAAAAAAAAAAAA3195560.23576464597742305No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAA3124880.23054995898118946No Hit
GTAGTCACAGCGAAAAAAAAAAAAAAAAAAA3047070.22480922899849368No Hit
CTGAGGCCCAAAAAAAAAAAAAAAAAAAAAA2971460.21923081241975537No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760120.20363839660504104No Hit
CATTTACGAGACCCGAAAAAAAAAAAAAAAA2732240.20158144310398002No Hit
GTGCCACTCAAAAAAAAAAAAAAAAAAAAAA2416980.17832193231687468No Hit
TACGATGGGACAAAAAAAAAAAAAAAAAAAA2373490.17511329143591542No Hit
TGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA2170770.16015684904943442No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2099840.15492371734820565No Hit
TACGATGGGACAATCAAAAAAAAAAAAAAAA2063920.15227358213450007No Hit
CATCATCTTAAAAAAAAAAAAAAAAAAAAAA1870810.1380261542080333No Hit
CGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA1861830.13736362040460687No Hit
GGGTTCAGGACAAAAAAAAAAAAAAAAAAAA1859850.13721753834104516No Hit
GGACTGACAAAAAAAAAAAAAAAAAAAAAAA1815990.13398159929669307No Hit
TCAACTCACAAAAAAAAAAAAAAAAAAAAAA1809920.1335337618594104No Hit
GTACCAAAAAAAAAAAAAAAAAAAAAAAAAA1798780.13271186580482577No Hit
TTCTCAGATGTAAAAAAAAAAAAAAAAAAAA1792980.13228394864893792No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1787730.1318966098440394No Hit
TTGCTCGAAAAAAAAAAAAAAAAAAAAAAAA1637200.12079068407234947No Hit
GCACCTCAAAAAAAAAAAAAAAAAAAAAAAA1599960.11804316081626938No Hit
TCGCCCAAAAAAAAAAAAAAAAAAAAAAAAA1587300.11710912095531413No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1562790.11530080207758796No Hit
ACATGTGTCGTTTCAAAAAAAAAAAAAAAAA1504560.11100466139011367No Hit
ACCTTAGCTGGGCTACAAAAAAAAAAAAAAA1386110.10226556016340357No Hit
GCACCTCATAAAAAAAAAAAAAAAAAAAAAA1358920.1002595140481292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG775950.024.8978182
GTGGCGC332800.024.720211
TCGCTAC398850.024.7110671
TGGGCCG277950.024.6278761
GGCGCCT521150.024.604342
ACGGGTA156000.024.5778162
GCGCCTG685450.024.5695253
GAGGCGA112250.024.5561355
ATGGCGC452900.024.522621
GGGCCGT231600.024.4789542
GTGCGCA132050.024.4716153
ACGGGCG155450.024.4628035
ACGCGGC117100.024.4252194
GGCCGTA232100.024.3931373
GTCGCTA203600.024.3816223
ATTTACG463500.024.3552232
GCATTTC1063550.024.329433
TCGCCCA583300.024.3198181
TCGGGCA162950.024.3035744
TCCCGCA286500.024.2991984
Produced by FastQC (version 0.11.5)