FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006127214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142625200
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTAAACATTAACCATCCGTAGTTATTTTTGAGACATGGATTAAAAC10867020.7619284670591172No Hit
GATCAGGGAGCTAAATGACTTGCCAAAAGTCACCCAGTTATTGAGGTCTG8953540.6277670425703171No Hit
GATCTGAACAGGTGGCAAAGGCTTAACAGGCTAGCGGTCACTGTAGTGAC8132830.5702239155492859No Hit
GATCTTTCCACTGCACTGTGCCGCCTCGGTGAACAGATGCGGGGAATTTA6581540.4614570216203027No Hit
GATCATCAAATTTAACTTTCTAAATCCTAAGGAGGAAACCGAGACTCCAG5038670.35328048619739005No Hit
GATCACTCCCTCCGTCTTTTCGCCGCTGTGGGGCCCCAGCCCGAGAGGAG4848330.33993501849603014No Hit
GATCTTCCAATCCTTTCCTCCACAAAGATAGAAGTTAATTTTGCTGCATC4622330.324089291373474No Hit
GATCTGGGAAAGCTATATAACTGCGAAAGCCTTGTCACATCTACAAATTC4621430.324026188920331No Hit
GATCCCGAGACTCTCGGCTTGCGCAGGCGCTCTTTGGTGTTTACAGCGGC4267010.299176442872648No Hit
GATCCTTTCTTTCCATGGTTTGGCTTCTACTCCAAAGCCACTTATTTGTG4262040.2988279771036254No Hit
GATCTCTGTTCCCTATGGGAGCAGTGAATCACCTGAACTTTGGGTCAATG4252510.2981597922386787No Hit
GATCTTCTAAAAGGCCGTGACATCACGGCCCAGGTGACCGCGGCCCAGCC4103600.287719140796998No Hit
GATCGATTTGGGATTTTAAATGGAAATGCACTAAAAATTCCTTAATTCCA3888290.2726229305901061No Hit
GATCTTCAAGGGGTGAAGGGCATCTCGACTGGGGCCACCCCCAAGCTCGC3690810.25877685009381235No Hit
GATCTGGGACTTGGTTGGGGCCTCGGCCGGTCCAGCTGTCTGGCTGCGCA3555590.2492960570782723No Hit
GATCATGTCCTGTTCTCGCCAACATCACAAGATGTCCCCAGACACGCCGC3441770.24131570017079731No Hit
GATCTTTAAAAAACAACGAATCGCAAAAAGTAACATTTAACAGGCCAGAG3146090.22058444089824236No Hit
GATCTGGCGCTGCCCTAGGGTCAGGGGCCCAGCAATAGTCAGGACCCCAA3111710.2181739271881827No Hit
GATCCCCCAGAGCAGCCTGAGGCTTCCCACAGGCAGCCAGGCAGCCTCCG3095140.2170121409119847No Hit
GATCAAAGGGGATAATGTGTGAGGAAGCATGTTAAAAGCTGAAAGTTTTG3061440.21464930461096635No Hit
GATCCCAGGGCTGGGTGGGGCCAGGCTGTCCCATTCCCCAACACTCCTCC3056930.2143330912068835No Hit
GATCCTTCGACCACACGCGCCGCTCGGTCACCTCAGAGAGCGTGGCGGGG2809330.19697290520889715No Hit
GATCCCCGGCGGGCCGCTTCTCCCCGCAGGCCGTGTAGCGTCAGATACGG2726520.19116677838138No Hit
GATCGCCGCCGTTGTTGGGCTGTGGAGACGTCTCAACCAAAGTGGAGCCG2691080.18868194400428537No Hit
GATCCTGCTAGTTATAGTAAGGGATGAATTTGTTGAGCAGGGCAAGTTTA2666110.18693120149875336No Hit
GATCTCACAGTAAAACCACAGGAACCAGAGAGCGATATGACATCTCTGCT2583100.1811110519038711No Hit
GATCCTGAAACTTGTTTTGAGGGGAGAGCAGAGAGGAAAGGGGTTGTTGG2539860.17807932959953782No Hit
GATCGCCTTTAACGTCGGTAACTCTGGAGTGTGAAATCAGTCATCCTTCT2533120.17760676233933415No Hit
GATCGCCGGGCCCTCCCGGTTCCCCGGGCCAAGCACACGCCGAACGGGAA2529770.17737188098596882No Hit
GATCATGGCTCGGTACAGCGCGGCATTACAGGCCAGGAGATGCCCACCCG2520420.1767163166116507No Hit
GATCAGCGAGTCCAGCGCCGGGCGGTCCAGACACGTGAAGTTCTGCAACA2504020.17556644968771298No Hit
GATCAAGCCGGGCACTTTCCACCGTGACATTTATCCTGTACTTTTGAAAA2459830.17246811923839545No Hit
GATCTTCTCGTCGTCCGAGTCGGGGTCCAGCGGCTTGTTGTACTCGTCGT2435550.17076575528027305No Hit
GATCCCAGACCCCCTATCCTTGCTGGGAGACACAACCCAGAACTCTGCAT2423340.16990966533263407No Hit
GATCCTGTTGCGGGGTTCGAGCCGGGGCACGGGAGGCCGACCCGAGGCCG2407990.16883341793736312No Hit
GATCCCCTGGGAAGGTGGCCACTCACCTGCGCTCTGAAATAGAAGAACAG2401690.16839170076536264No Hit
GATCCTGGCTCGCGTCGAGCTCGACGAGGGCCGGAGTTGCGGACGAGACG2386450.1673231658921425No Hit
GATCAAGTCCCAGCCTCAACTGCATACACTTTCTAATACAGCCGCGCCCA2302210.1614167762779649No Hit
GATCGAAATCCGGAGGAACGGAGTAGTCTGGGAGCGGAGATTATTGACAG2285100.16021712852988113No Hit
GATCTCTTCGGGCCACCGTCGCTGAGCATGCGCAAATAAACGTGGCGGGA2242020.15719662443943988No Hit
GATCATTTGCAACTAAAAAACAGCGGAGGCTGAAAGACGTAGGTTTCCGG2154410.15105395119516046No Hit
GATCCGGTTGCCTTTCTGCGGTGGAGGAGGAAGTGTTGGATTTCAGCGCT2088330.14642082885773341No Hit
GATCTGTTCTGTTTCACCATGTAACACACAATACATGCATGCATTGTATT2024060.14191461256496046No Hit
GATCCGCCGGCTGGGAATCGCGGGTCCCCGCGCGTCCTGCAGCACTTCCC1960940.13748902718453682No Hit
GATCTTGGCAGGCCAGTGGGGGAGACACGCAGGAGGCCTGGAAGCCCCTG1930320.13534214150094093No Hit
GATCTGTTCTGTTTCACCATGTAACACACAATACATGCATGCACTGTATT1875010.13146414518612418No Hit
GATCGGAATGGTGGAGAACTTCAACCAGGCACTCAAGGTGGGCCATACTC1842490.1291840432125599No Hit
GATCTGGTAAGGTGGATGGTGGGAGGCTCGAGGGTGTCCAGAGCCTGATG1804690.12653374018055716No Hit
GATCTTCTTGATGTCTTCAGCTTGCTTTTTCCAGGAGGAGCTGCTCTCGC1742460.12217055611490817No Hit
GATCCCCCAGGGAGGCAGCAGAGACACAGTGACCCCGCTCCCCACCAGAC1625680.11398266225042979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)