FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006127215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142625200
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCAGGGAGCTAAATGACTTGCCAAAAGTCACCCAGTTATTGAGGTCTG9722060.6816509284474273No Hit
GATCTGAACAGGTGGCAAAGGCTTAACAGGCTAGCGGTCACTGTAGTGAC7733250.5422078286305646No Hit
GATCTGGGAAAGCTATATAACTGCGAAAGCCTTGTCACATCTACAAATTC6859510.4809465648426786No Hit
GATCATCAAATTTAACTTTCTAAATCCTAAGGAGGAAACCGAGACTCCAG6014940.42173052167499153No Hit
GATCATGTCCTGTTCTCGCCAACATCACAAGATGTCCCCAGACACGCCGC5863540.4111152867796154No Hit
GATCCTAAACATTAACCATCCGTAGTTATTTTTGAGACATGGATTAAAAC5821150.4081431612365837No Hit
GATCGCCGCCGTTGTTGGGCTGTGGAGACGTCTCAACCAAAGTGGAGCCG5739440.40241415962957455No Hit
GATCTTTCCACTGCACTGTGCCGCCTCGGTGAACAGATGCGGGGAATTTA5438820.38133653800310185No Hit
GATCTCTGTTCCCTATGGGAGCAGTGAATCACCTGAACTTTGGGTCAATG5091370.3569754853980923No Hit
GATCCCCTGGGAAGGTGGCCACTCACCTGCGCTCTGAAATAGAAGAACAG4960490.34779898643437485No Hit
GATCTTCTAAAAGGCCGTGACATCACGGCCCAGGTGACCGCGGCCCAGCC4427110.31040166814840575No Hit
GATCTTCCAATCCTTTCCTCCACAAAGATAGAAGTTAATTTTGCTGCATC4424340.3102074528203992No Hit
GATCCCAGACCCCCTATCCTTGCTGGGAGACACAACCCAGAACTCTGCAT4281990.3002267481482936No Hit
GATCTTGGCAGGCCAGTGGGGGAGACACGCAGGAGGCCTGGAAGCCCCTG4171790.2925002033301268No Hit
GATCTTTAAAAAACAACGAATCGCAAAAAGTAACATTTAACAGGCCAGAG4152110.29112036302140154No Hit
GATCTGGCGCTGCCCTAGGGTCAGGGGCCCAGCAATAGTCAGGACCCCAA3977210.27885745296062686No Hit
GATCGATTTGGGATTTTAAATGGAAATGCACTAAAAATTCCTTAATTCCA3859190.2705826179384849No Hit
GATCGAAATCCGGAGGAACGGAGTAGTCTGGGAGCGGAGATTATTGACAG3362020.2357241216839661No Hit
GATCTTCAAGGGGTGAAGGGCATCTCGACTGGGGCCACCCCCAAGCTCGC3332270.23363823503840833No Hit
GATCGCCGGGCCCTCCCGGTTCCCCGGGCCAAGCACACGCCGAACGGGAA3270430.2293023953691213No Hit
GATCCTGAAACTTGTTTTGAGGGGAGAGCAGAGAGGAAAGGGGTTGTTGG3176450.22271309698426367No Hit
GATCAAGCCGGGCACTTTCCACCGTGACATTTATCCTGTACTTTTGAAAA3092880.21685368364075913No Hit
GATCTGGGACTTGGTTGGGGCCTCGGCCGGTCCAGCTGTCTGGCTGCGCA3081770.21607471891362814No Hit
GATCCCCCAGAGCAGCCTGAGGCTTCCCACAGGCAGCCAGGCAGCCTCCG2967900.20809085631431193No Hit
GATCCTTCGACCACACGCGCCGCTCGGTCACCTCAGAGAGCGTGGCGGGG2893620.202882800514916No Hit
GATCAGCGAGTCCAGCGCCGGGCGGTCCAGACACGTGAAGTTCTGCAACA2893590.20288069709981124No Hit
GATCGCCTTTAACGTCGGTAACTCTGGAGTGTGAAATCAGTCATCCTTCT2883930.2022033974360772No Hit
GATCAAGTCCCAGCCTCAACTGCATACACTTTCTAATACAGCCGCGCCCA2789280.1955671227805465No Hit
GATCAAAGGGGATAATGTGTGAGGAAGCATGTTAAAAGCTGAAAGTTTTG2779250.19486388099718704No Hit
GATCTCACAGTAAAACCACAGGAACCAGAGAGCGATATGACATCTCTGCT2730160.19142199274742472No Hit
GATCCTTTCTTTCCATGGTTTGGCTTCTACTCCAAAGCCACTTATTTGTG2719010.19064022346682075No Hit
GATCCGGTTGCCTTTCTGCGGTGGAGGAGGAAGTGTTGGATTTCAGCGCT2648590.18570280707757114No Hit
GATCACTTCTTTCCACTGAGTCTCTGGGAGGAGACAGGGAGATGCCAGAA2441430.1711780246408068No Hit
GATCACTCCCTCCGTCTTTTCGCCGCTGTGGGGCCCCAGCCCGAGAGGAG2415320.1693473523612938No Hit
GATCATGGCTCGGTACAGCGCGGCATTACAGGCCAGGAGATGCCCACCCG2397200.16807688963801629No Hit
GATCCCCGGCGGGCCGCTTCTCCCCGCAGGCCGTGTAGCGTCAGATACGG2355270.16513701645992435No Hit
GATCCTGTTGCGGGGTTCGAGCCGGGGCACGGGAGGCCGACCCGAGGCCG2323900.16293754539870936No Hit
GATCATTTGCAACTAAAAAACAGCGGAGGCTGAAAGACGTAGGTTTCCGG2212430.1551219560077742No Hit
GATCCTGCTAGTTATAGTAAGGGATGAATTTGTTGAGCAGGGCAAGTTTA2172810.1523440457927491No Hit
GATCCCAGGGCTGGGTGGGGCCAGGCTGTCCCATTCCCCAACACTCCTCC2165230.15181258290961205No Hit
GATCTGTTCTGTTTCACCATGTAACACACAATACATGCATGCATTGTATT2148320.15062695792889333No Hit
GATCTGTTCTGTTTCACCATGTAACACACAATACATGCATGCACTGTATT2076610.1455990946901389No Hit
GATCCCCCAGGGAGGCAGCAGAGACACAGTGACCCCGCTCCCCACCAGAC2033350.14256597010906907No Hit
GATCTGGTAAGGTGGATGGTGGGAGGCTCGAGGGTGTCCAGAGCCTGATG2033150.14255194734170398No Hit
GATCGGAATGGTGGAGAACTTCAACCAGGCACTCAAGGTGGGCCATACTC2017030.14142171229207742No Hit
GATCCGGGTTAGGGGGGCGCAAAACCCCATCCGCCCATTTCCGCACCAAC1949960.13671917725619315No Hit
GATCTCTTCGGGCCACCGTCGCTGAGCATGCGCAAATAAACGTGGCGGGA1866080.13083802862327273No Hit
GATCCCGAGACTCTCGGCTTGCGCAGGCGCTCTTTGGTGTTTACAGCGGC1856610.13017405058853557No Hit
GATCTTCTTGATGTCTTCAGCTTGCTTTTTCCAGGAGGAGCTGCTCTCGC1824970.12795564879137766No Hit
GATCCGCCGGCTGGGAATCGCGGGTCCCCGCGCGTCCTGCAGCACTTCCC1680330.11781438343294172No Hit
GATCCCACTGGGCTCGTGGAGGGCTGGTGAGGGGCAGAGCCCCATCCAGA1514020.10615375123049783No Hit
GATCTATGTCCCTCGCCCAGACCGACGGCTGGCCCCAGAAGCCGAAACGA1505980.10559003598242106No Hit
GATCAGATGGGCTTCAGGGGCCTGGAGCCTTGTCGCCCACGCCAGGGCTA1471730.10318863707114873No Hit
GATCCTGGCTCGCGTCGAGCTCGACGAGGGCCGGAGTTGCGGACGAGACG1454900.10200862119737605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)