FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006127319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803320755
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTAT1529117855.454666032321371TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTATGCCGT129976460.46365175932213293TruSeq Adapter, Index 7 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTATGCC115571080.4122649175762978TruSeq Adapter, Index 7 (96% over 33bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTA62354710.22243159256315642TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACA36783850.1312152736514092TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)