FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006127320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803320755
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1669795535.9564911614975005Illumina Single End PCR Primer 1 (100% over 50bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA117488260.419103878107591Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC67302470.24008123180324403Illumina Single End PCR Primer 1 (100% over 40bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG40902760.14590824088554932Illumina Single End PCR Primer 1 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)