FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006127321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729214393
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT781785992.8645092595332797TruSeq Adapter, Index 15 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATGCCGT205708620.7537283275641878TruSeq Adapter, Index 15 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATG48783730.17874641920811532TruSeq Adapter, Index 15 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATGCC36468800.13362380065683613TruSeq Adapter, Index 15 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)