FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006127329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728677181
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTAT1136151094.163743142322265TruSeq Adapter, Index 25 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTATGCCGT390984701.432872685425957TruSeq Adapter, Index 25 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTATGCC98357550.3604587258796005TruSeq Adapter, Index 25 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTATG37928730.13900042945387903TruSeq Adapter, Index 25 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)