FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006127337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993049498
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT1125572163.760619932119813TruSeq Adapter, Index 1 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTATGCCGT349401391.1673759162134645TruSeq Adapter, Index 1 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTATGCC102664970.34301126683204625TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTATG84455620.2821724801291609TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)